Mass Spectrometry

Please use the CIF iLab Portal to request training and schedule time on instruments.

The Mass Spec Lab has a eight mass spectrometers with a variety of ionization techniques available for molecular mass measurements of small molecules, biomolecules (e.g. proteins, polysaccharides) and nano materials (e.g. gold nano clusters).

The facility offers a wide range of services, including (1) accurate mass analysis for generating precise chemical formulae and MS/MS for further structural elucidation; (2) targeted quantitative analysis by triple quad MS; (3) intact protein analysis; and (4) targeted and untargeted metabolite and small molecule screening in biological samples. We also provide method development services. Requests for full service analysis of samples can be done through iLab. Please accommodate your samples with a Mass Spec Submission Form listed to the right.

These rates are for CSU Affiliates and are subject to revision. Please contact Dr. Karolien Denef or Dr. Claudia Boot about Mass Spec Analysis.

Walk-up Instruments (Self-service)

Agilent 5973N Mass Selective Detector interfaced to a 6890 gas chromatograph which is equipped with a 7683 automatic liquid sampler.  This instrument is a single quadrupole mass spectrometer utilizing an electron ionization source (EI).

Instrument Use:  $24/hr

Thermo-Finnigan LTQ LC/MS-MS. This instrument is a linear ion trap mass spectrometer with electrospray ionization (ESI). Allows MSn scanning for multi-stage MS experiments to probe the structure of ions.

Sample Run:  $12/sample

The DART source (IonSense DART-SVP) is interfaced with our Bruker MaXis QTOF. DART permits rapid, noncontact, high-resolution, exact mass measurements of compounds directly in gases, liquids, and solids. A unique application is the direct detection of chemicals on surfaces without any sample preparation requirements and with mass spectra generated within seconds.

Sample Run:  $8/sample

Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by flow injection. Available sources include: dualESI and Multimode.

Sample Run: $12/sample

Quantitative Targeted Analyses (Self-service)

Thermo Scientific TSQ 8000 Evo Triple Quadrupole GC-MS/MS, equipped with automatic liquid sampler or Markes Thermal Desorption. Available sources are EI and CI (methane). Default GC column is a Phenomenex ZB-5HT-MS Inferno (30m x 0.25mm x 0.25um)

GC-MS/MS: $40/hr or $400/day (>10h)

Markes Thermal Desorption: $45/hr or $625/day (>10h)

Waters Xevo UPLC-MS/MS tandem quadrupole with ESI ionization and Waters Acquity H-Class UPLC

Instrument Use:  $60/hr or $600/day

Method Development:  $250/day

Accurate-Mass Analyses (CIF operated)

Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by UPLC, flow injection or syringe infusion. Available sources include: dualESI and Multimode.

Direct Injection / syringe infusion: $20/sample

UPLC: $100/h or $1000/day

Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by HPLC, flow injection or syringe infusion. This instrument can be used for MS/MS analysis. Available sources include: dualESI and Multimode.

Direct Injection / syringe infusion: $20/sample

UPLC: $100/h or $1000/day (>10h)

Used for ultra-high-resolution (70,000), high mass-accuracy mass measurements with typically less than +/-2 ppm mass measurement error. Equipped with Waters Acquity HClass UPLC and UV detector. This instrument can be used for MS/MS analysis in data-dependent (DDA) and data-independent acquisition (DIA) mode. Sample introduction can be done by UPLC, flow injection or syringe infusion. Available sources include: dualESI and APCI.

Direct Injection or Syringe Infusion:  $20/sample

UPLC:  $100/hr or $1000/day (>10h)

*DART:  $20/sample

Karolien Denef

Dr. Karolien Denef
Associate Director/Research Scientist III
Lab Manager, Mass Spectrometry and Spectroscopy
CIF, Chemistry, C1F
(970) 491-3832
Karolien.Denef@colostate.edu

Claudia Boot

Dr. Claudia Boot
Research Scientist II
Mass Spectrometry Scientist
CIF, Chemistry, C1A
(970) 491-7049
Claudia.Boot@colostate.edu