The Mass Spec Lab has a eight mass spectrometers with a variety of ionization techniques available for molecular mass measurements of small molecules, biomolecules (e.g. proteins, polysaccharides) and nano materials (e.g. gold nano clusters).
The facility offers a wide range of services, including (1) accurate mass analysis for generating precise chemical formulae and MS/MS for further structural elucidation; (2) targeted quantitative analysis by triple quad MS; (3) intact protein analysis; and (4) targeted and untargeted metabolite and small molecule screening in biological samples. We also provide method development services. Requests for full service analysis of samples can be done through iLab. Please accommodate your samples with a Mass Spec Submission Form listed under “Resources”.
Walk-up Instruments (Self-service)
- MALDI-TOF: The Bruker Microflex LRF is a time-of-flight mass spectrometer with Matrix-Assisted Laser Desorption Ionization, a soft ionization technique ideal for polymers, peptides, intact proteins and a wide variety of compounds. The sample is mixed with a matrix (small organic molecule that absorbs at the laser’s wavelength) in a common solution. 1 uL of the sample is spotted on the plate and allowed to dry which cause the matrix to crystallize and the analyte is embedded in the matrix. A nitrogen laser is used on the sample spot which causes the desorption and ionization event. The ions are analyzed by time of flight mass analyzer. The Microflex offers high resolution (reflectron mode for compounds less than 5,000 Da) and low resolution (linear mode for compounds greater than 5,000 Da).
- GC/MS: Agilent 5973N Mass Selective Detector interfaced to a 6890 gas chromatograph which is equipped with a 7683 automatic liquid sampler. This instrument is a single quadrupole mass spectrometer utilizing an electron ionization source (EI).
- Ion-trap LC/MS-MS: Thermo-Finnigan LTQ LC/MS-MS. This instrument is a linear ion trap mass spectrometer with electrospray ionization (ESI). Allows MSn scanning for multi-stage MS experiments to probe the structure of ions.
- Direct Analysis in Real Time: The DART source (IonSense DART-SVP) is interfaced with our Bruker MaXis QTOF. DART permits rapid, noncontact, high-resolution, exact mass measurements of compounds directly in gases, liquids, and solids. A unique application is the direct detection of chemicals on surfaces without any sample preparation requirements and with mass spectra generated within seconds.
- Agilent 6224 TIME OF FLIGHT (TOF) LC-MS (Walkup W-TOF): Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by flow injection. Available sources include: dualESI and Multimode.
Quantitative Targeted Analyses (Self-service)
- GC triple quadrupole MS: Thermo Scientific TSQ 8000 Evo Triple Quadrupole GC-MS/MS, equipped with automatic liquid sampler or Markes Thermal Desorption. Available sources are EI and CI (methane). Default GC column is a Phenomenex ZB-5HT-MS Inferno (30m x 0.25mm x 0.25um)
- LC triple quadrupole MS:Waters Xevo UPLC-MS/MS tandem quadrupole with ESI ionization and Waters Acquity H-Class UPLC
Accurate-Mass Analyses (CIF operated)
- Agilent 6230 TIME OF FLIGHT (TOF) LC-MS B-TOF: Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by UPLC, flow injection or syringe infusion. Available sources include: dualESI and Multimode.
- Agilent 6520 TIME OF FLIGHT (TOF) LC-MS Q-TOF: Used for accurate mass measurements with typically less than +/-2 ppm mass measurement error. Sample introduction can be done by HPLC, flow injection or syringe infusion. This instrument can be used for MS/MS analysis. Available sources include: dualESI and Multimode.
- Bruker MAXIS Q-TOF LC-MS/MS: Used for ultra-high-resolution (70,000), high mass-accuracy mass measurements with typically less than +/-2 ppm mass measurement error. Equipped with Waters Acquity HClass UPLC and UV detector. This instrument can be used for MS/MS analysis in data-dependent (DDA) and data-independent acquisition (DIA) mode. Sample introduction can be done by UPLC, flow injection or syringe infusion. Available sources include: dualESI and APCI.
Please use the iLab Portal to request training and schedule time on instruments.
These rates are for CSU Affiliates and are subject to revision. Please contact Dr. Karolien Denef or Dr. Claudia Boot about Mass Spec Analysis.