Electron Microscopy

The Electron Microscopy Lab is a research and service facility, serving the biological and materials research communities at CSU. It is open to university, state, and outside users. It houses one scanning electron microscope and two transmission electron microscopes to provide detailed imaging of surfaces, thin films, and nanostructures. Microstructural crystallographic analysis via electron diffraction and elemental analysis via energy dispersive spectroscopy are available.

The facility offers a wide range of services, including (1) imaging of thin films or nanoparticles at atomic resolution using the 2100F TEM and secondary electron imaging (SEI) and/or backscattered electron imaging (BSE) of surfaces at nm resolution in the SEM, (2) crystallographic analysis from thin samples using various modes of electron diffraction in the 2100F TEM and electron backscatter diffraction (EBSD) from bulk crystalline samples in the SEM, (3) spatially-resolved elemental analysis via energy dispersive spectroscopy (EDS) in the SEM and in the scanning transmission (STEM) mode in the JEOL 2100F TEM, (4) and e-beam lithography in the SEM. The JEOL JEM-1400 TEM has imaging features similar to the 2100F, but is used mainly to image biological samples, such as sections of tissues or cells and negatively stained particles, at lower resolution. The 1400 TEM does not have EDS capability.

Sample preparation tools include gold or carbon coating, sectioning and embedding soft matter and biological specimens, and a saw, grinder, and a variety of mechanical polishing wheels (for further information go to https://www.research.colostate.edu/iss/sample-preparation-tools/).

Instrument use and technician services are available on a recharge fee basis. Users can be trained in order to operate the microscopes by themselves. Alternatively, they can request assistance from the staff. User training on the 6500 SEM requires a few hours with a staff member and training on the 2100F TEM requires enrolling in an 8-week course with Dr. Roy Geiss. Training on the 1400 TEM is handled by Dr. Royer.

Please use the iLab Portal to request training or services and access the instrument calendars.

JEOL JSM-6500 Field Emission Scanning Electron Microscope

The SEM is equipped with the In-Lens thermal field emission electro gun (TFEG). This high-resolution tool has a multi-purpose specimen chamber and a motorized, automated stage. The SEM is equipped with an Oxford Instruments energy dispersive X-ray spectrometer (EDS) and uses Oxford Aztec software for qualitative and quantitative elemental analysis. It also has a Nabity e-beam lithography patterning system (NPGS). The NPGS allows for lithographic patterns to be written with our SEM a few tens of nanometers in width. There is a solid-state BSE detector for BS electron imaging, a special holder for STEM imaging of thin films and an EDAX EBSD camera for diffraction.

JEOL JEM-2100F Transmission Electron Microscope (80 – 200 kV)

The TEM is equipped with several specimen holders, including (1) a single-tilt holder with ± 20° tilt about the x-axis (rod axis), (2) a double-tilt holder with ± 20° tilt about both the x- and y-axes, (3) and a beryllium double-tilt holder with ± 20° tilt about both the x-axis and y-axes, specifically for low background EDS analysis. There is also a special high tilt holder that provides ± 40° about the x-axis using special samples and can be used for tomography and microED analysis.

This TEM has an UHR objective lens pole piece that provides 0.195 nm TEM image resolution and 0.135 nm STEM resolution.  TEM images and diffraction patterns are captured with a Gatan Ultrascan 2K x 2K CCD camera. For STEM imaging there are three detectors: (1) a JEOL bright-field (BF) STEM detector, (2) a JEOL annular dark field (ADF) detector, and (3) a Gatan ADF detector. There is an Oxford Instruments SSD EDS detector with Aztec software for elemental analysis.

*User training on the TEM requires enrolling in an 8-week, extensive course with Dr. Roy Geiss.

JEOL JEM-1400 120 keV Transmission Electron Microscope

Equipped with several different specimen holders (a single specimen holder, ±26°; a high tilt specimen holder, ±70°; a Fischione dual axis Tomography holder, ±70°; and a Gatan liquid nitrogen-cooled specimen holder, ±70°), an Orius fastscan digital camera, and a Gatan Ultrascan high-resolution digital camera.  Direct magnification range on this TEM is between 50x and 1,200,000x.

JEOL JEM-2000 EXII Transmission Electron Microscope

This TEM is equipped with a ±60° tilting (goniometer) stage, operates at accelerating voltages from 80kV to 200kV, and has a magnification range from 50x to 500,000x. It is capable of selected area diffraction from 100 to 2,500mm. Two specimens can be loaded simultaneously. Images are currently captured on film, which is processed daily.

Please use the iLab Portal to view pricing for instrument usage.

A Guide to Scanning Microscope Observation from JEOL

Williams & Carter: Transmission Electron Microscopy: A Textbook for Materials Science (textbook and online access available via CSU Library)

EDS in the TEM Explained from Oxford Instruments


Coming soon!

Scientific Staff:

Dr. Roy Geiss: microscopy expert, contact for services and training on the JEOL 2100F TEM and JEOL JSM-5800 FESEM

Dr. Rebecca Miller: contact for services and training on the JEOL JSM-5800 FESEM

Dr. Suzanne Royer: contact for services and training on the JEOL JEM-1400 TEM