Total Internal Reflection Microscope
The Nikon Total Internal Reflection Fluorescence (TIRF) Inverted Microscope (Eclipse Ti) has perfect focus control, a four laser line launch (405, 488, 561 and 640 nm) and an Intensilight Hg illumination source. A motorized XY Piezo Z stage and stage-top environmental chamber with temperature, CO2/air, humidity capability, will support culture dishes, multi-well plates or slides. Equipped with 20X, 40X, 60X DIC objectives and a 100X TIRF objective. Microscope has an Andor Clara camera for wide field imaging and an Andor iXon3 EMCCD camera for TIRF imaging. Microscope has built-in N-STORM super-resolution imaging capability and is networked to a RAID array for multiple terabyte storage. Nikon elements software controls all aspects of acquisition and analysis with a deconvolution software package included.