Typhoon Trio Imager
The Typhoon Trio is available to CSU researchers for imaging of fluorescent and radioactive experiments.
The Typhoon Trio is located in Microbiology B319 and is open during normal working hours. If you wish to be trained on how to use the Typhoon, please contact John Anderson or Carol Wilusz for free instruction.
Phosphorimaging
Phosphor storage screens are available for communal use. Please treat them with care – they cost over $1000 each. Following exposure to the screen, the images are visualized by scanning in storage phosphor mode at 200mM resolution. Screens are available for general (32P, 35S) and tritium isotopes.
Fluorescent Imaging
The Typhoon has three lasers enabling excitation and visualization of a wide range of wavelengths (link to Table for scanning info). Applications include fluorescent stained protein gels (SYPRO Ruby, Deep Purple), fluorescent western blots (ECL Plus, ECL Plex), fluorescent nucleic acid stains (SYBR-green), 2D-DIGE (Cy2, Cy3, Cy5) etc. Gels and blots are positioned directly on a glass platen for scanning.
The Typhoon is not recommended for straight chemiluminescence, but fluorescent alternatives such as ECL Plus, and Cy-dye labeled secondary antibodies do allow western blotting experiments to be analyzed. Please ask as we may have samples available for you to test.
Downstream Applications
Images can be saved as TIFF files for manipulation in standard graphic software. Analysis is performed using ImageQuantTL software which is loaded on the Typhoon computer.
User Fees and Access
Although the Typhoon has been funded entirely by a Shared Instrumentation Grant from the NIH, user fees are necessary to cover the costs of the service contract and consumables. Actual fees will be adjusted depending on the number of major users and overall use of the machine.
Use time is tracked based on the number of minutes logged on to computer that controls the Typhoon. For this reason it will be considerably cheaper if you perform the majority of data analysis on your own computer. The ImageQuant software to do this is available upon request. Access to the instrument outside of normal hours working can be arranged – contact John or Carol.
0-49 minutes: $3.25/minute
50-699 minutes: $162.50/month
700-1499 minutes: $300/month
>1500 minutes: $440/month