{"id":14,"date":"2025-11-05T20:10:17","date_gmt":"2025-11-05T20:10:17","guid":{"rendered":"https:\/\/www.research.colostate.edu\/arc\/?page_id=14"},"modified":"2026-03-04T23:41:53","modified_gmt":"2026-03-04T23:41:53","slug":"electron-microscopy","status":"publish","type":"page","link":"https:\/\/www.research.colostate.edu\/arc\/services\/electron-microscopy\/","title":{"rendered":"Electron Microscopy"},"content":{"rendered":"\n
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Access Advanced Electron Microscopy Capabilities<\/h2>\n\n\n\n
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The Electron Microscopy Laboratory at ARC offers high\u2011resolution imaging and analysis for researchers in the physical sciences, life sciences, engineering, agriculture, and other fields. Open to university, state, and external users, the lab features SEM and TEM instruments for detailed surface, thin\u2011film, and nanoscale characterization of materials and biological samples.<\/p>\n<\/div>\n\n\n\n

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roy geiss – materials<\/a><\/div>\n\n\n\n
Bradley Guilliams – biological TEM<\/a><\/div>\n\n\n\n
Rebecca Miller – sem<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n
<\/div>\n\n\n\n

Services<\/h2>\n\n\n\n
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  • High-Resolution Imaging of Materials<\/strong>:<\/strong> Atomic-resolution imaging of thin films and nanoparticles using TEM.<\/li>\n\n\n\n
  • High-Resolution Imaging of Biological Samples<\/strong>: Ultrastructural imaging of cells,  biological tissues, extracellular vesicles, viruses, phages, etc.<\/li>\n\n\n\n
  • Crystallographic Analysis:<\/strong> Structural characterization through electron diffraction in TEM and electron backscatter diffraction (EBSD) for bulk crystalline samples in SEM.<\/li>\n\n\n\n
  • Elemental Analysis:<\/strong> Spatially resolved energy dispersive X-ray spectroscopy (EDS) for precise elemental mapping in SEM and in scanning transmission mode (STEM) in TEM.<\/li>\n\n\n\n
  • Cathodoluminescence (CL) Imaging:<\/strong> Optical characterization of materials providing insights into electronic structure, defects, and composition in semiconductors, minerals, and other luminescent materials.<\/li>\n\n\n\n
  • E-Beam Lithography:<\/strong> Nanopatterning capabilities using SEM for advanced fabrication applications.<\/li>\n\n\n\n
  • Materials Sample Preparation<\/strong>:<\/strong> Coating services (gold, chromium, carbon), mechanical cutting and  polishing tools.<\/li>\n\n\n\n
  • Biological Sample Preparation:<\/strong> Fixation, dehydration, critical point dying, staining (uranyl acetate, osmium tetroxide), embedding, sectioning.<\/li>\n\n\n\n
  • Air-free Sample Handling:<\/strong> Direct transfer of SEM sample from glovebox to SEM exchange chamber.<\/li>\n<\/ul>\n\n\n\n

    Users can be trained to operate the instruments independently or request assistance from expert staff. Training requirements vary by instrument, with SEM training taking a few hours and TEM training available through structured courses.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Instruments<\/h2>\n\n\n\n

    JEOL JSM-IT800(HL) Field Emission SEM<\/strong><\/p>\n\n\n\n

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    • Detectors:<\/strong> Secondary electron (SED), Upper in-lens (UED), Retractable backscatter (VBED), STEM<\/li>\n\n\n\n
    • Analytical Tools:<\/strong> EDS (Oxford Ultim Max 170 mm\u00b2), EBSD (Oxford C-Nano+), CL (Deben Centaurus)<\/li>\n\n\n\n
    • Special Features:<\/strong> Gentle beam mode, low vacuum for insulating samples, e-beam lithography (NPGS)<\/li>\n\n\n\n
    • Sample Capacity:<\/strong> Up to 4-inch diameter, ~30 mm height, air-free sample transfer<\/li>\n<\/ul>\n\n\n\n

      JEOL JEM-2100F Transmission Electron Microscope (80-200 kV)<\/strong><\/strong><\/strong><\/p>\n\n\n\n

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      • Resolution:<\/strong> 0.195 nm TEM, 0.135 nm STEM<\/li>\n\n\n\n
      • Detectors:<\/strong> TEM (Gatan Ultrascan CCD), STEM (BF, ADF)<\/li>\n\n\n\n
      • Specimen Holders:<\/strong> Single-tilt, double-tilt, high-tilt (for tomography\/microED), low-background EDS<\/li>\n\n\n\n
      • Analytical Tools:<\/strong> EDS (Oxford SSD, Aztec software)<\/li>\n<\/ul>\n\n\n\n

        EM Sample Preparation Tools<\/strong><\/strong><\/strong><\/strong><\/p>\n\n\n\n

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        • Quorum Q150T ES Plus Coater:<\/strong> Metal sputtering and carbon evaporation for conductive coatings, with high vacuum capability and film thickness control.<\/li>\n\n\n\n
        • Pace Technologies PICO 155P Precision Cutter:<\/strong> High-accuracy wafering saw for sectioning metals, ceramics, and electronics (\u22640.5 cm thick).<\/li>\n\n\n\n
        • Pace Technologies NANO 2000T Grinder-Polisher:<\/strong> Dual-wheel system for grinding and polishing various materials.<\/li>\n\n\n\n
        • Pace Technologies Giga-0900 Vibratory Polisher:<\/strong> Adjustable frequency polisher for simultaneous preparation of multiple samples.<\/li>\n\n\n\n
        • Plasma Cleaner:<\/strong> Removes hydrocarbons and modifies TEM substrates using oxygen or argon plasma.<\/li>\n\n\n\n
        • Reichert-Jung Ultracut E Ultramicrotomes<\/strong> \u2013 for cutting thick and ultrathin resin sections, on either glass or diamond knifes, for TEM or SEM. <\/li>\n<\/ul>\n\n\n\n
          <\/div>\n\n\n\n
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          \"JEOL
          JEOL JSM-IT800(HL) Field Emission Scanning Electron Microscope<\/figcaption><\/figure>\n\n\n\n
          \"JEOL
          JEOL JEM-2100F Transmission Electron Microscope (80 \u2013 200 kV)<\/figcaption><\/figure>\n\n\n\n
          \"\"
          Reichert-Jung Ultramicrotome<\/figcaption><\/figure>\n\n\n\n
          \"\"
          Quorum Q150T Coater<\/figcaption><\/figure>\n<\/figure>\n<\/div>\n<\/div>\n\n\n\n

          <\/p>\n","protected":false},"excerpt":{"rendered":"

          Access Advanced Electron Microscopy Capabilities The Electron Microscopy Laboratory at ARC offers high\u2011resolution imaging and analysis for researchers in the physical sciences, life sciences, engineering, agriculture, and other fields. Open to university, state, and external users, the lab features SEM and TEM instruments for detailed surface, thin\u2011film, and nanoscale characterization of materials and biological samples. […]<\/p>\n","protected":false},"author":6,"featured_media":0,"parent":36,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"microsite":[],"class_list":["post-14","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/pages\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":0,"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/pages\/36"}],"wp:attachment":[{"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/media?parent=14"}],"wp:term":[{"taxonomy":"microsite","embeddable":true,"href":"https:\/\/www.research.colostate.edu\/arc\/wp-json\/wp\/v2\/microsite?post=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}